Sorghum pp 141-151 | Cite as

Identification and Quantification of Carotenoids and Tocochromanols in Sorghum Grain by High-Performance Liquid Chromatography

  • Darwin Ortiz
  • Mario G. FerruzziEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1931)


Carotenoids and tocochromanols are lipid-soluble secondary plant metabolites that are essential for the normal functioning of plants and, in some cases function as a source of vitamin A and E in humans. Enhancement of the provitamin A carotenoid and tocochromanols levels in sorghum and other cereal grains through traditional breeding and transgenic methods has increased in recent years with interest in biofortification of grains to combat micronutrient deficiencies in developing countries. With this increase in research, reliable methodology for the extraction, identification, and quantification of individual carotenoids and tocochromanols species from sorghum and other cereal grains is essential. Here, we describe a basic method for extraction of carotenoid and tocochromanols adapted to sorghum grain and chromatographic condition for separation, identification, and quantification of individual carotenoid using High-Performance Liquid Chromatography (HPLC) system.

Key words

Carotenoids Vitamin A Vitamin E Lutein Zeaxanthin β-carotene Tocopherols Tocotrienols 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Food SciencePurdue UniversityWest LafayetteUSA
  2. 2.Department of AgronomyPurdue UniversityWest LafayetteUSA
  3. 3.Department of Food, Bioprocessing and Nutrition Sciences, Plants for Human Health InstituteNorth Carolina State UniversityKannapolisUSA

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