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GapmeR-Mediated Gene Silencing in Motile T-Cells

  • Mobashar Hussain Urf Turabe Fazil
  • Seow Theng Ong
  • Madhavi Latha Somaraju Chalasani
  • Atish Kizhakeyil
  • Navin Kumar Verma
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1930)

Abstract

Gene silencing is an important method to study gene functions in health and diseases. While there are various techniques that are applied to knockdown specific gene(s) of interest, they have certain limitations in application to T-lymphocytes. T-cells are “hard-to-transfect” cells and are recalcitrant to transfection reagents. Here, we describe the use of novel cell-permeating antisense molecules, called “GapmeR”, to knockdown specific gene(s) in human primary T-cells.

Key words

GapmeR RNAi Gene knockdown T-Lymphocytes 

Notes

Acknowledgements

This work was supported in part by grants from Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant to N.K.V. and the Singapore Ministry of Education under its Singapore Ministry of Education (MOE) Academic Research Fund (AcRF) Tier 2 Grant (MOE2017-T2-2-004). A.K. acknowledges Ph.D. fellowship provided by Lee Kong Chian School of Medicine, Nanyang Technological University Singapore.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Mobashar Hussain Urf Turabe Fazil
    • 1
  • Seow Theng Ong
    • 1
  • Madhavi Latha Somaraju Chalasani
    • 1
    • 2
  • Atish Kizhakeyil
    • 1
  • Navin Kumar Verma
    • 1
  1. 1.Lee Kong Chain School of MedicineNanyang Technological University SingaporeSingaporeSingapore
  2. 2.Autoimmunity and Inflammation ProgramHospital for Special SurgeryNYUSA

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