Computational Analysis of Protein–Protein Interactions in Motile T-Cells

  • Sunil Kumar
  • Mobashar Hussain Urf Turabe Fazil
  • Khurshid Ahmad
  • Minaketan Tripathy
  • Jagath Chandana Rajapakse
  • Navin Kumar Verma
Part of the Methods in Molecular Biology book series (MIMB, volume 1930)


Analysis of protein–protein interactions is important for better understanding of molecular mechanisms involved in immune regulation and has potential for elaborating avenues for drug discovery targeting T-cell motility. Currently, only a small fraction of protein–protein interactions have been characterized in T-lymphocytes although there are several detection methods available. In this regard, computational approaches garner importance, with the continued explosion of genomic and proteomic data, for handling protein modeling and protein–protein interactions in large scale. Here, we describe a computational method to identify protein–protein interactions based on in silico protein design.

Key words

Protein modeling Docking Protein–protein interactions Molecular dynamics simulation 



This work was supported in part by grants from Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant to N.K.V., the Singapore Ministry of Education (MOE) under its MOE Academic Research Fund (AcRF) Tier 1 (2014-T1-001-141) and MOE-AcRF Tier 2 (MOE2017-T2-2-004). J.C.R. acknowledges funding support from the MOE-AcRF Tier 2 grant (MOE2016-T2-1-029).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sunil Kumar
    • 1
    • 2
  • Mobashar Hussain Urf Turabe Fazil
    • 3
  • Khurshid Ahmad
    • 1
  • Minaketan Tripathy
    • 4
  • Jagath Chandana Rajapakse
    • 5
  • Navin Kumar Verma
    • 6
  1. 1.ICAR-NBAIMMauIndia
  2. 2.Institute of Life SciencesBhubaneswarIndia
  3. 3.Lymphocyte Signalling Research Laboratory, Lee Kong Chain School of MedicineNanyang Technological University SingaporeSingaporeSingapore
  4. 4.Laboratory of Fundamentals of Pharmaceutics, Faculty of PharmacyUniversiti Teknologi MARA (UiTM)Bandar Puncak AlamMalaysia
  5. 5.School of Computer Science and EngineeringNanyang Technological University SingaporeSingaporeSingapore
  6. 6.Lee Kong Chain School of MedicineNanyang Technological University SingaporeSingaporeSingapore

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