Immunometabolomic Phenotyping of Motile T-Cells

  • Madhavi Latha Somaraju Chalasani
  • Gaelin Looi Wen Dong
  • Navin Kumar Verma
Part of the Methods in Molecular Biology book series (MIMB, volume 1930)


The immune system and its components defend our body against diverse pathogens and help in maintaining tissue homeostasis. Immune cells are highly dynamic in terms of their growth, migration, differentiation, and effector functions, and adopt diverse metabolic configurations to respond to varying immunological challenges. Growing body of evidence suggests that metabolic pathways fuel immune cells for their functioning, including T-cell migration to the site of infection. This chapter provides detailed methodology for the efficient extraction of T-cell metabolites for successful downstream immunometabolomic profiling of motile T-lymphocytes.

Key words

Immunometabolism Mass-spectrometry LFA-1 T-cell migration 



The authors acknowledge Professor Jeremy Everett, Nanyang Technological University Singapore for reviewing this chapter. This work was supported in part by grants from Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant and the Singapore Ministry of Education (MOE) under its MOE Academic Research Fund (AcRF) Tier 1 (2014-T1-001-141) and MOE-AcRF Tier 2 (MOE2017-T2-2-004) to N.K.V.


  1. 1.
    O'Neill LA, Kishton RJ, Rathmell J (2016) A guide to immunometabolism for immunologists. Nat Rev Immunol 16:553–565CrossRefGoogle Scholar
  2. 2.
    Almeida L, Lochner M, Berod L, Sparwasser T (2016) Metabolic pathways in T cell activation and lineage differentiation. Semin Immunol 28:514–524CrossRefGoogle Scholar
  3. 3.
    Kishore M, Cheung KCP, Fu H, Bonacina F, Wang G, Coe D, Ward EJ, Colamatteo A, Jangani M, Baragetti A, Matarese G, Smith DM, Haas R, Mauro C, Wraith DC, Okkenhaug K, Catapano AL, De Rosa V, Norata GD, Marelli-Berg FM (2017) Regulatory T cell migration is dependent on glucokinase-mediated glycolysis. Immunity 47:875–889.e10CrossRefGoogle Scholar
  4. 4.
    Frauwirth KA, Riley JL, Harris MH, Parry RV, Rathmell JC, Plas DR, Elstrom RL, June CH, Thompson CB (2002) The CD28 signaling pathway regulates glucose metabolism. Immunity 16:769–777CrossRefGoogle Scholar
  5. 5.
    Parry RV, Chemnitz JM, Frauwirth KA, Lanfranco AR, Braunstein I, Kobayashi SV, Linsley PS, Thompson CB, Riley JL (2005) CTLA-4 and PD-1 receptors inhibit T-cell activation by distinct mechanisms. Mol Cell Biol 25:9543–9553CrossRefGoogle Scholar
  6. 6.
    Verma NK, Dempsey E, Long A, Davies A, Barry SP, Fallon PG, Volkov Y, Kelleher D (2012) Leukocyte function-associated antigen-1/intercellular adhesion molecule-1 interaction induces a novel genetic signature resulting in T-cells refractory to transforming growth factor-β signaling. J Biol Chem 287:27204–27216CrossRefGoogle Scholar
  7. 7.
    Volkov Y, Long A, McGrath S, Ni Eidhin D, Kelleher D (2001) Crucial importance of PKC-beta(I) in LFA-1-mediated locomotion of activated T cells. Nat Immunol 2:508–514CrossRefGoogle Scholar
  8. 8.
    Plassmeier J, Barsch A, Persicke M, Niehaus K, Kalinowski J (2007) Investigation of central carbon metabolism and the 2-methylcitrate cycle in Corynebacterium glutamicum by metabolic profiling using gas chromatography-mass spectrometry. J Biotechnol 130:354–363CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Madhavi Latha Somaraju Chalasani
    • 3
    • 1
  • Gaelin Looi Wen Dong
    • 2
  • Navin Kumar Verma
    • 4
  1. 1.Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of MedicineNanyang Technological University SingaporeSingaporeSingapore
  2. 2.Singapore Phenome Center, Lee Kong Chian School of MedicineNanyang Technological University SingaporeSingaporeSingapore
  3. 3.Autoimmunity and Inflammation ProgramHospital for Special SurgeryNYUSA
  4. 4.Lee Kong Chian School of MedicineNanyang Technological University SingaporeSingaporeSingapore

Personalised recommendations