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Immunometabolomic Phenotyping of Motile T-Cells

  • Madhavi Latha Somaraju Chalasani
  • Gaelin Looi Wen Dong
  • Navin Kumar Verma
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1930)

Abstract

The immune system and its components defend our body against diverse pathogens and help in maintaining tissue homeostasis. Immune cells are highly dynamic in terms of their growth, migration, differentiation, and effector functions, and adopt diverse metabolic configurations to respond to varying immunological challenges. Growing body of evidence suggests that metabolic pathways fuel immune cells for their functioning, including T-cell migration to the site of infection. This chapter provides detailed methodology for the efficient extraction of T-cell metabolites for successful downstream immunometabolomic profiling of motile T-lymphocytes.

Key words

Immunometabolism Mass-spectrometry LFA-1 T-cell migration 

Notes

Acknowledgments

The authors acknowledge Professor Jeremy Everett, Nanyang Technological University Singapore for reviewing this chapter. This work was supported in part by grants from Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant and the Singapore Ministry of Education (MOE) under its MOE Academic Research Fund (AcRF) Tier 1 (2014-T1-001-141) and MOE-AcRF Tier 2 (MOE2017-T2-2-004) to N.K.V.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Madhavi Latha Somaraju Chalasani
    • 3
    • 1
  • Gaelin Looi Wen Dong
    • 2
  • Navin Kumar Verma
    • 4
  1. 1.Lymphocyte Signalling Research Laboratory, Lee Kong Chian School of MedicineNanyang Technological University SingaporeSingaporeSingapore
  2. 2.Singapore Phenome Center, Lee Kong Chian School of MedicineNanyang Technological University SingaporeSingaporeSingapore
  3. 3.Autoimmunity and Inflammation ProgramHospital for Special SurgeryNYUSA
  4. 4.Lee Kong Chian School of MedicineNanyang Technological University SingaporeSingaporeSingapore

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