Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults
In recent years there have been numerous attempts to establish reliable and consistent noninvasive biomarkers of gut inflammation. Here we describe the development of an enzyme-linked immunoassay for the measurement of the pro-inflammatory, calcium-binding protein S100A12 in fecal samples.
Key wordsELISA S100A12 Calcium EF-hand Fecal extraction Inflammatory Biomarker
We would like to acknowledge the contribution of Professor Carolyn Geczy as this fecal assay is based on the detection of serum S100A12 assay developed in her laboratory.