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Enzyme-Linked Immunosorbent Assay to Measure S100A12 in Fecal Samples of Children and Adults

  • Steven T. Leach
  • Andrew S. DayEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1929)

Abstract

In recent years there have been numerous attempts to establish reliable and consistent noninvasive biomarkers of gut inflammation. Here we describe the development of an enzyme-linked immunoassay for the measurement of the pro-inflammatory, calcium-binding protein S100A12 in fecal samples.

Key words

ELISA S100A12 Calcium EF-hand Fecal extraction Inflammatory Biomarker 

Notes

Acknowledgment

We would like to acknowledge the contribution of Professor Carolyn Geczy as this fecal assay is based on the detection of serum S100A12 assay developed in her laboratory.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Women’s and Children’s HealthUniversity of New South Wales MedicineSydneyAustralia
  2. 2.Department of PediatricsUniversity of Otago (Christchurch)ChristchurchNew Zealand

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