Targeted Mass Spectrometry of S100 Proteins

  • Juan Martínez-Aguilar
  • Mark P. MolloyEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1929)


The S100 protein family has attracted great interest in the field of biomarker research, and a growing number of studies reveal dysregulation of many of the 21 S100 protein isoforms in various human diseases. In cancer, S100 protein expression has been associated with tumor growth, progression, and response to treatment. Some S100 proteins are also considered candidate therapeutic targets. From an analytical perspective, multiplexed analysis of the family-wide S100 protein expression is challenging due to their relatively small size and high-sequence identity. Here we describe a mass spectrometry method using selected reaction monitoring which enables the targeted, multiplexed detection and quantitation of the entire S100 protein family in cell lines and tissue samples.

Key words

S100 proteins Ca2+-binding EF-hand Biomarkers Quantitative proteomics Targeted mass spectrometry Selected reaction monitoring 



MPM acknowledges support from the Cancer Institute NSW and Sydney Vital Translational Cancer Research Centre.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Red de Apoyo a la Investigación-Universidad Nacional Autónoma de México-INCMNSZMexico CityMexico
  2. 2.Bowel Cancer and Biomarker Research, Kolling Institute, Royal North Shore HospitalThe University of SydneySt. LeonardsAustralia

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