Expression and Characterization of MICU2, a Ca2+ Sensor Protein

  • Wenping Wu
  • Jimin ZhengEmail author
  • Zongchao JiaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1929)


MICU2 is a Ca2+ sensor protein of mitochondrial uniporter which is a highly selective Ca2+ channel mediating mitochondrial Ca2+ uptake to regulate cell death, metabolism, and cytoplasmic Ca2+ signaling. Here we describe the procedures for protein preparation of various MICU2 constructs, which have enabled successful in vitro characterizations of MICU2 including interaction with MICU1 using pull-down assays and oligomerization using multi-angle laser light scattering.

Key words

MICU2 Ca2+ sensor EF hand Mitochondrial SEC MALLS Pull-down Purification 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.College of ChemistryBeijing Normal UniversityBeijingChina
  2. 2.Department of Biochemical and Molecular ScienceQueen UniversityKingstonCanada

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