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Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells

  • Debanti Sengupta
  • Guillem PratxEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1928)

Abstract

Radionuclide imaging with cellular-scale resolution allows characterization of biological processes and metabolic fluxes in single live cells. In this protocol, we describe how to image radiotracer uptake with single-cell resolution and compare the method to conventional bulk-scale gamma counting. We describe the utility of both techniques, give examples where each technique is recommended, and provide detailed side-by-side instructions for both techniques.

Key words

Single-cell analysis Cancer metabolism Radiotracer quantitation Microscopy 

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Medical Physics, Department of Radiation OncologyStanford UniversityStanfordUSA

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