Radioluminescence Microscopy: A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells

  • Debanti Sengupta
  • Guillem PratxEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1928)


Radionuclide imaging with cellular-scale resolution allows characterization of biological processes and metabolic fluxes in single live cells. In this protocol, we describe how to image radiotracer uptake with single-cell resolution and compare the method to conventional bulk-scale gamma counting. We describe the utility of both techniques, give examples where each technique is recommended, and provide detailed side-by-side instructions for both techniques.

Key words

Single-cell analysis Cancer metabolism Radiotracer quantitation Microscopy 


  1. 1.
    Sengupta D, Pratx G (2016) Single-cell characterization of FLT uptake with radioluminescence microscopy. J Nucl Med 57(7):1136–1140. Scholar
  2. 2.
    Pratx G, Chen K, Sun C, Martin L, Carpenter CM, Olcott PD, Xing L (2012) Radioluminescence microscopy: measuring the heterogeneous uptake of radiotracers in single living cells. PLoS One 7(10):e46285CrossRefGoogle Scholar
  3. 3.
    Natarajan A, Türkcan S, Gambhir SS, Pratx G (2015) Multiscale framework for imaging radiolabeled therapeutics. Mol Pharm 12(12):4554–4560CrossRefGoogle Scholar
  4. 4.
    Rauscher I, Eiber M, Souvatzoglou M, Schwaiger M, Beer AJ (2014) PET/MR in oncology: non–18F-FDG tracers for routine applications. J Nucl Med 55(Suppl 2):25S–31SCrossRefGoogle Scholar
  5. 5.
    Kim TJ, Türkcan S, Pratx G (2017) Modular low-light microscope for imaging cellular bioluminescence and radioluminescence. Nat Protoc 12(5):1055–1076CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Medical Physics, Department of Radiation OncologyStanford UniversityStanfordUSA

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