Recombinant Protein Production in Yeast pp 309-322 | Cite as
Guidelines for Small-Scale Production and Purification of Hepatitis B Surface Antigen Virus-Like Particles from Recombinant Pichia pastoris
Protocol
First Online:
Abstract
Virus-like particle (VLP)-based vaccines have been in the market since decades for preventing viral infection and have proven their usefulness also in other areas of biotechnology. Here, we describe in detail simple small-scale production and purification procedures for the generation of hepatitis B surface antigen (HBsAg) VLPs using Pichia pastoris as expression host. This protocol may also be applicable with variations to other HBsAg-based VLPs additionally carrying antigens of other pathogens.
Key words
HBsAg Pichia pastoris Vaccine Virus Virus-like particleNotes
Acknowledgments
We are grateful to C. Gurramkonda for critical comments.
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