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Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells

  • Yasuhiro Yoshimura
  • Atsuhiro Taguchi
  • Ryuichi NishinakamuraEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

Nephrons, the functional units of the kidney, are derived from nephron progenitor cells (NPCs). Here, we describe methods to reconstruct nephron tissue via induction of NPCs from mouse and human pluripotent stem cells, which mimic multistep developmental signals in vivo. Induced NPCs differentiate into three-dimensional nephron structures, including glomerular podocytes and nephric tubules, which are useful for studying early stages of kidney specification and morphogenetic processes in the context of normal development or disease.

Key words

Kidney Nephron Regeneration Pluripotent stem cells Mouse Human Nephron progenitor cells Growth factors Flow cytometry 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yasuhiro Yoshimura
    • 1
  • Atsuhiro Taguchi
    • 2
  • Ryuichi Nishinakamura
    • 1
    Email author
  1. 1.Department of Kidney Development, Institute of Molecular Embryology and GeneticsKumamoto UniversityKumamotoJapan
  2. 2.Department of Genome RegulationMax Planck Institute for Molecular GeneticsBerlinGermany

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