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Dissociation of Tissues for Single-Cell Analysis

  • Andrew S. Potter
  • S. Steven PotterEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

It is now a routine to carry out single-cell RNA-Seq to define the gene expression patterns of thousands of cells, thereby revolutionizing many areas of research. Projects are underway to use these techniques to create an atlas of the expressed genes in all cell types of the human body. Here we describe cold-active protease methods for single-cell dissociation of organs and tissues that better preserve the in vivo gene expression patterns.

Key words

Organ dissociation Single-cell suspensions Psychrophilic proteases Reduced gene expression artifacts Immediate early response genes 

Notes

Acknowledgments

This work was supported by NIH UO1 HL 122642 and UO1 DK107350 (SSP).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division of Developmental BiologyCincinnati Children’s Medical CenterCincinnatiUSA

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