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Analysis of the Teashirt Target Genes in Ureteric Bud Development

  • Laurent FasanoEmail author
  • Irene Sanchez-Martin
  • Xavier Caubit
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

Microarrays and RNA-seq (RNA sequencing) are powerful techniques to assess transcript abundance in biological samples and to improve our understanding of the relationship between genotype and phenotype. Tshz3+/− heterozygous mouse is a model for a human 19q12 syndrome characterized by autistic traits and renal tract defects (Caubit et al., Nat Genet 48:1359–1369, 2016). To unravel renal tract pathological mechanisms, we took advantage of Tshz3 mouse and performed comparative genome-wide expression profiling on embryonic ureter and/or kidney.

Key words

Microarray RNA-seq Mouse Ureter Kidney Tshz3 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Laurent Fasano
    • 1
    Email author
  • Irene Sanchez-Martin
    • 1
  • Xavier Caubit
    • 1
  1. 1.Aix Marseille University, CNRS, IBDMMarseilleFrance

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