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Optical Projection Tomography Imaging to Study Kidney Organogenesis

  • Renata Prunskaite-HyyryläinenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

Optical projection tomography (OPT) is a 3D imaging technology. The 3D tomographic reconstruction permits precise analysis and quantification of various structures in developing embryonic tissues and adult organs of small rodents or biopsies. OPT enables detailed and accurate studies of kidney organogenesis, namely, ureteric tree branching morphogenesis and nephron quantification.

Key words

Optical projection tomography Phenotyping 3D imaging Branching morphology Nephron quantification Whole-mount immunostaining Whole-mount in situ hybridization 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Biochemistry and Molecular MedicineUniversity of OuluOuluFinland

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