Decellularizing and Recellularizing Adult Mouse Kidneys
Decellularization is the process by which resident cells are lysed and cellular debris is removed from the tissue, leaving behind the extracellular matrix scaffold. The extracellular matrix scaffold can be used for three-dimensional culturing of cells. Here we describe methods of decellularizing whole and thick sections of mouse kidneys using a 0.1% sodium dodecyl sulfate (SDS) detergent solution and strategies to repopulate whole and thick sections of decellularized mouse kidneys with cells.
Key wordsMouse kidney dissection Cannulation and perfusion Kidney decellularization Extracellular matrix Kidney recellularization Bioreactor Three-dimensional (3D) tissue culture
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