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Time-Lapse Technologies and 4D Imaging of Kidney Development

  • Ulla SaarelaEmail author
  • Ilya SkovorodkinEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1926)

Abstract

Time-lapse imaging is a technique of frequent imaging and following a course of a process. Because the development of the embryonic kidney can proceed ex vivo after dissection, it is possible to study the morphogenesis by culturing the kidney in the onstage incubator of a microscope and follow the developmental process by imaging. Confocal microscopes and other three-dimensional imaging systems offer the possibility for tracking the development process in four dimensions—3D and the time.

Key words

Time-lapse imaging Kidney development Tissue culture 

Notes

Acknowledgments

This work was supported financially by the Suomen Akatemia (Academy of Finland) (206038, 121647, 250900, 260056; Centre of Excellence grant 2012-2017 251314), Munuaissäätiö—Finnish Kidney and Liver Association, the Sigrid Juséliuksen Säätiö, Novo Nordisk, Syöpäjärjestöt (Cancer Society of Finland), the European Community’s Seventh Framework Programme (FP7/2007-2013; grant FP7-HEALTH-F5-2012-INNOVATION-1 EURenOmics 305608), and H2020 Marie Skłodowska-Curie Actions Innovative Training Network “RENALTRACT” Project ID 642937.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Faculty of Biochemistry and Molecular MedicineUniversity of OuluOuluFinland
  2. 2.Laboratory of Developmental Biology, Biocenter OuluInfoTechOuluFinland
  3. 3.Oulu Center for Cell Matrix ResearchOuluFinland

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