Ca2+-Dependent Regulation of TFEB and Lysosomal Function
Lysosomes are emerging as calcium store organelles that can modulate various intracellular processes such as the regulation of nutrient signaling through the activation of TFEB, a master gene for lysosomal function, or very specialized functions like lysosomal exocytosis. Here, we describe two different techniques that can be used to study these processes. In the case report, we described two studies where these methodologies allowed us to unravel the role of calcineurin in the dephosphorylation of TFEB as well as the involvement of TFEB in lysosomal exocytosis, respectively.
Key wordsLysosomes Ca2 + TFEB Calcineurin Lysosomal exocytosis
This work was supported by grants from the ML4 Foundation (SDP and DLM) and Cure Sanfilippo Foundation (DLM) and by EU Horizon 2020, BATCure 666918 (DLM).
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