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TRPML1-/TFEB-Dependent Regulation of Lysosomal Exocytosis

  • Simone Di Paola
  • Diego L. MedinaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1925)

Abstract

Emerging experimental evidences indicate that the lysosome can trigger a calcium signaling, via TRPML1/calcineurin/TFEB pathway, that promotes lysosomal exocytosis and clearance of lysosomal accumulation in various cellular models of lysosomal storage disorders (LSDs). Here, we described methods to determine TFEB activation and lysosomal exocytosis that may represent innovative tools to study lysosomal function and to develop novel therapeutic approaches to promote clearance in LSDs.

Key words

Lysosomal calcium TFEB Autophagy Calcineurin LSDs 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Telethon Institute of Genetics and Medicine (TIGEM)PozzuoliItaly

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