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Imaging Intracellular Ca2+ in Cardiomyocytes with Genetically Encoded Fluorescent Probes

  • Antonio Campo
  • Marco MongilloEmail author
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1925)

Abstract

Calcium (Ca2+) is a key player in cardiomyocyte homeostasis, and its roles span from excitation-contraction coupling to metabolic and structural signaling. Alterations in the function or expression of Ca2+-handling proteins are common findings in failing cardiomyocytes, which have been linked to impaired contractility and detrimental remodeling of the cellular structure. For these reasons, the study of intracellular Ca2+ handling in cardiomyocytes represents a central method in experimental molecular cardiology.

Key words

Calcium probes Cardiomyocyte Calcium transients Excitation-contraction coupling Calcium-induced calcium release 
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Notes

Acknowledgments

The authors are grateful to Dr. Giulia Borile for her theoretical and technical help to develop and improve this protocol.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biomedical SciencesUniversity of PaduaPaduaItaly
  2. 2.Venetian Institute of Molecular MedicinePaduaItaly
  3. 3.Neuroscience Institute, Italian National Research Council (CNR)PaduaItaly

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