Phototropism pp 121-130 | Cite as
A Simple Procedure to Observe Phototropic Responses in the Red Seaweed Pyropia yezoensis
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Abstract
The marine red seaweed Pyropia yezoensis exhibits phototropic responses in gametophyte and conchosporangia phases, but not in sporophytes. These responses are easily monitored with a simple culturing box that has one side open to allow for unilateral light irradiation within an incubator. Confirmation of phototropic responses is achieved by changing the direction of unilateral light irradiation via rotation of the culture dishes clockwise 90°.
Key words
Conchosporangium Gametophyte Phototropism Pyropia yezoensis Red seaweed SporophyteNotes
Acknowledgments
We are grateful to the Marine Resources Research Center, Aichi Fisheries Research Institute for kindly providing P. yezoensis strain U-51. This work was supported in part by KAKENHI (15H04539).
References
- 1.Goyal A, Szarzynska B, Fankhauser C (2013) Phototropism: at the crossroads of light-signaling pathways. Trends Plant Sci 18:393–401CrossRefGoogle Scholar
- 2.Fankhauser C, Christie JM (2015) Plant phototropic growth. Curr Biol 25:R384–R389CrossRefGoogle Scholar
- 3.Hohm T, Preuten T, Fankhauser C (2013) Phototropism: translating light into directional growth. Am J Bot 100:47–59CrossRefGoogle Scholar
- 4.Liscum E, Askinosie SK, Leuchtman DL, Morrow J, Willenburg KT, Coats DR (2014) Phototropism: growing towards an understanding of plant movement. Plant Cell 26:38–55CrossRefGoogle Scholar
- 5.Buggeln RG (1974) Negative phototropism of the chapter of Alaria esculenta (Laminariales). J Phycol 10:80–82Google Scholar
- 6.Kataoka H (1975) Phototropism in Vaucheria germinate I. The action spectrum. Plant Cell Physiol 16:427–437Google Scholar
- 7.Kataoka H (1975) Phototropism in Vaucheria germinate II. The mechanism of bending and branching. Plant Cell Physiol 16:439–448Google Scholar
- 8.Waaland SD, Nehlsen W, Waaland JR (1977) Phototropism in red alga, Griffithsia pacifica. Plant Cell Physiol 18:603–612Google Scholar
- 9.Ishizawa K, Wada S (1979) Growth and phototropic bending in Boergesenia rhizoid. Plant Cell Physiol 20:973–982Google Scholar
- 10.Ishizawa K, Wada S (1979) Action spectrum of negative phototropism in Boergesenia forbesii. Plant Cell Physiol 20:983–987Google Scholar
- 11.Rico JM, Guiry MD (1996) Phototropism in seaweeds: a review. Sci Mar 60:273–281Google Scholar
- 12.Takahashi F, Yamagata D, Ishikawa M, Fukamatsu Y, Ogura Y, Kasahara M, Kiyosue T, Kikuyama M, Wada M, Kataoka H (2007) AUREOCHROME, a photoreceptor required for photomorphogenesis in stramenopiles. Proc Natl Acad Sci U S A 104:19625–19630CrossRefGoogle Scholar
- 13.Takahashi F (2016) Blue-light-regulated transcription factor, Aureochrome, in photosynthetic stramenopiles. J Plant Res 129:189–197CrossRefGoogle Scholar
- 14.Kim JK, Yarish C, Hwang EK, Park M, Kim Y (2017) Seaweed aquaculture: cultivation technologies, challenges and its ecosystem service. Algae 32:1–13CrossRefGoogle Scholar
- 15.Mikami K, Li L, Takahashi M (2012) Monospore-based asexual life cycle in Porphyra yezoensis. In: Mikami K (ed) Porphyra yezoensis: Frontiers in physiological and molecular biological research. Nova Science, New York, pp 15–37Google Scholar
- 16.Nakamura Y, Sasaki N, Kobayashi M, Ojima N, Yasuike M, Shigenobu Y, Satomi M, Fukuma Y, Shiwaku K, Tsujimoto A, Kobayashi T, Nakayama I, Ito F, Nakajima K, Sano M, Wada T, Kuhara S, Inouye K, Gojobori T, Ikeo K (2013) The first symbiont-free genome sequence of marine red alga, Susabi-nori (Pyropia yezoensis). PLoS One 8:e57122. https://doi.org/10.1371/journal.pone.0057122CrossRefPubMedPubMedCentralGoogle Scholar
- 17.Wang L, Mao Y, Kong F, Li G, Ma F, Zhang B, Sun P, Bi G, Zhang F, Xue H, Cao M (2013) Complete sequence and analysis of plastid genomes of two economically important red algae: Pyropia haitanensis and Pyropia yezoensis. PLoS One 8:e65902. https://doi.org/10.1371/journal.pone.0065902CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Kong F, Sun P, Cao M, Wang L, Mao Y (2014) Complete mitochondrial genome of Pyropia yezoensis: reasserting the revision of genus Porphyra. Mitochondrial DNA 25:335–336CrossRefGoogle Scholar
- 19.Van Tussenbrock BI (1984) Effect of continuous unilateral irradiation on the conchocelis of Porphyra umbilicalis (L.) J. Ag. and some other red algae. J Exp Mar Biol Ecol 83:263–274CrossRefGoogle Scholar
- 20.Migita S, Kim CP (1970) Studies on horizontal growth of Conchocelis. Bull Fac Fish Nagasaki Univ 30:1–8 in Japanese with English abstractGoogle Scholar
- 21.Takahashi M, Mikami K (2016) Phototropism in the marine red macroalgae Pyropia yezoensis. Am J Plant Sci 7:2412–2428CrossRefGoogle Scholar
- 22.Li L, Saga N, Mikami K (2008) Phosphatidylinositol 3-kinase activity and asymmetrical accumulation of F-actin are necessary for establishment of cell polarity in the early development of monospores from the marine red alga Porphyra yezoensis. J Exp Bot 59:3575–3586CrossRefGoogle Scholar
- 23.Li L, Saga N, Mikami K (2009) Ca2+ influx and phosphoinositide signalling are essential for the establishment and maintenance of cell polarity in monospores from the red alga Porphyra yezoensis. J Exp Bot 60:3477–3489CrossRefGoogle Scholar
- 24.Takahashi M, Saga N, Mikami K (2010) Photosynthesis-dependent extracellular Ca2+ influx triggers an asexual reproductive cycle in the marine red macroalgae Porphyra yezoensis. Am J Plant Sci 1:1–11CrossRefGoogle Scholar
- 25.Adams E, Mikami K, Shin R (2017) Selection and functional analysis of a Pyropia yezoensis ammonium transporter PyAMT1 in potassium deficiency. J Appl Phycol 29:2617–2626CrossRefGoogle Scholar
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