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Measurement of Phototropism of the Sporangiophore of Phycomyces blakesleeanus

  • Luis M. CorrochanoEmail author
  • Paul Galland
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)

Abstract

The giant sporangiophore, fruiting body, of the fungus Phycomyces blakesleeanus is a single cell that grows guided by several environmental signals, including light. The phototropic response has been investigated in detail. Three proteins, the components of a photoreceptor and transcription factor complex and a regulator of the signal transduction protein Ras, participate in the signal transduction pathway. We describe the basic methods for characterizing phototropic bending and the correlated elongation and rotation responses of the sporangiophore.

Key words

Phototropism Fungus Blue light Phycomyces Sporangiophore Growing zone Photoreceptor Threshold box Tropostat 

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departamento de Genética, Facultad de BiologíaUniversidad de SevillaSevillaSpain
  2. 2.Fachbereich BiologiePhilipps-Universität MarburgMarburgGermany

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