Assays of Protonemal Growth Responses in Physcomitrella patens Under Blue- and Red-Light Stimuli

  • Sho Miyazaki
  • Masatoshi Nakajima
  • Hiroshi KawaideEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)


The non-seed land plant Physcomitrella patens is a model species for developmental, cellular, and molecular biology studies in mosses and also for performing genetic analyses. Previously, it was shown that wild-type P. patens displays a unique photomorphogenetic behavior, in which chloronemal filaments grow in the opposite direction to a blue-light source. Here, we describe bioassay systems that can be used to study light avoidance responses as well as other aspects of photomorphogenetic regulation in P. patens grown under red- and blue-light sources.

Key words

Bryophyte Gibberellin Physcomitrella Photomorphogenesis Blue light Red light Moss Protonema Caulonema Chloronema 



Japan Society for the Promotion of Science (JSPS) KAKENHI grants 24380060/15H04492 to MN and HK and 16K18693 to SM; the Agricultural Chemical Research Foundation to SM; and Wada Kunko-kai Research Foundation to MN, supported this work.


  1. 1.
    Kami C, Lorrain S, Hornitschek P et al (2010) Light-regulated plant growth and development. Curr Top Dev Biol 91:29–66CrossRefGoogle Scholar
  2. 2.
    Schaefer DG, Zrÿd JP (2001) The moss Physcomitrella patens, now and then. Plant Physiol 127:1430–1438CrossRefGoogle Scholar
  3. 3.
    Imaizumi T, Kadota A, Hasebe M et al (2002) Cryptochrome light signals control development to suppress auxin sensitivity in the moss Physcomitrella patens. Plant Cell 14:373–386CrossRefGoogle Scholar
  4. 4.
    Zhao X, Yu X, Foo E et al (2007) A study of gibberellin homeostasis and cryptochrome-mediated blue light inhibition of hypocotyl elongation. Plant Physiol 145:106–118CrossRefGoogle Scholar
  5. 5.
    Hayashi K, Horie K, Hiwatashi Y et al (2010) Endogenous diterpenes derived from ent-kaurene, a common gibberellin precursor, regulate protonema differentiation of the moss Physcomitrella patens. Plant Physiol 153:1085–1097CrossRefGoogle Scholar
  6. 6.
    Hayashi K, Kawaide H, Notomi M et al (2006) Identification and functional analysis of bifunctional ent-kaurene synthase from the moss Physcomitrella patens. FEBS Lett 580:6175–6181CrossRefGoogle Scholar
  7. 7.
    Miyazaki S, Katsumata T, Natsume M et al (2011) The CYP701B1 of Physcomitrella patens is an ent-kaurene oxidase that resists inhibition by uniconazole-P. FEBS Lett 585:1879–1883CrossRefGoogle Scholar
  8. 8.
    Miyazaki S, Toyoshima H, Natsume M, Kawaide H et al (2014) Blue-light irradiation up-regulates the ent-kaurene synthase gene and affects the avoidance response of protonemal growth in Physcomitrella patens. Planta 240:117–124CrossRefGoogle Scholar
  9. 9.
    Miyazaki S, Nakajima M, Kawaide H (2015) Hormonal diterpenoids derived from ent-kaurenoic acid are involved in the blue-light avoidance response of Physcomitrella patens. Plant Signal Behav 10:e989046CrossRefGoogle Scholar
  10. 10.
    Ashton NW, Cove DJ (1977) The isolation and preliminary characterization of auxotrophic and analogue resistant mutants in the moss Physcomitrella patens. Mol Gen Genet 154:87–95CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sho Miyazaki
    • 1
  • Masatoshi Nakajima
    • 1
  • Hiroshi Kawaide
    • 2
    Email author
  1. 1.Department of Applied Biological Chemistry, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo-kuJapan
  2. 2.Institute of AgricultureTokyo University of Agriculture and TechnologyFuchuJapan

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