Assays of Protonemal Growth Responses in Physcomitrella patens Under Blue- and Red-Light Stimuli
The non-seed land plant Physcomitrella patens is a model species for developmental, cellular, and molecular biology studies in mosses and also for performing genetic analyses. Previously, it was shown that wild-type P. patens displays a unique photomorphogenetic behavior, in which chloronemal filaments grow in the opposite direction to a blue-light source. Here, we describe bioassay systems that can be used to study light avoidance responses as well as other aspects of photomorphogenetic regulation in P. patens grown under red- and blue-light sources.
Key wordsBryophyte Gibberellin Physcomitrella Photomorphogenesis Blue light Red light Moss Protonema Caulonema Chloronema
Japan Society for the Promotion of Science (JSPS) KAKENHI grants 24380060/15H04492 to MN and HK and 16K18693 to SM; the Agricultural Chemical Research Foundation to SM; and Wada Kunko-kai Research Foundation to MN, supported this work.