Advertisement

Phototropism pp 199-206 | Cite as

Grafting as a Tool for Study of Phototropism in Phycomyces

  • Atsushi MiyazakiEmail author
  • Tamotsu Ootaki
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)

Abstract

Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate. Here, we present a method of grafting sporangiophores from different mutants to obtain heterokaryotic regenerates at the graft union, based on the large size of the sporangiophore and its remarkable regeneration capability.

Key words

Complementation Fungi Grafting Phycomyces Sporangiophore 

References

  1. 1.
    Cerdá-Olmedo E, Lipson ED (1987) A biography of Phycomyces. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. CSH, New YorkGoogle Scholar
  2. 2.
    Eslava AP (1987) Genetics. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. CSH, New YorkGoogle Scholar
  3. 3.
    Suárez T, Orejas M, Eslava AP (1985) Isolation, regeneration, and fusion of Phycomyces blakesleeanus spheroplasts. Exp Mycol 9:203–211CrossRefGoogle Scholar
  4. 4.
    Suárez T, Orejas M, Arnau J, Torres-Martínez S (1987) Protoplast formation and fusion. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. CSH, New YorkGoogle Scholar
  5. 5.
    Fukui J, Choi KS, Miyazaki A, Ootaki T, Oikawa T (2001) Analysis of the formation of protoplasts and regeneration of cells in Phycomyces blakesleeanus. J Microbiol Biotechnol 11:72–78Google Scholar
  6. 6.
    Heisenberg M, Cerdá-Olmedo E (1968) Segregation of heterokaryons in the asexual cycle of Phycomyces. Mol Gen Genet 102:187–195CrossRefGoogle Scholar
  7. 7.
    Ootaki T (1987) Heterokaryon formation. In: Cerdá-Olmedo E, Lipson ED (eds) Phycomyces. CSH, New YorkGoogle Scholar
  8. 8.
    Ootaki T, Miyazaki A, Choi KS (2002) Positional differentiation for development along the sporangiophore in Phycomyces blakesleeanus. Mycoscience 43:239–245CrossRefGoogle Scholar
  9. 9.
    Ootaki T (1973) A new method for heterokaryon formation in Phycomyces. Mol Gen Genet 121:49–56CrossRefGoogle Scholar
  10. 10.
    Gruen HE, Ootaki T (1970) Regeneration on Phycomyces sporangiophores. I. Induction by decapitation and basal cutting on sporangiophores attached to mycelium and after isolation. Can J Bot 48:55–65CrossRefGoogle Scholar
  11. 11.
    Ootaki T, Gruen HE (1970) Regeneration on Phycomyces sporangiophores. II. Regeneration on isolated segments from different positions along the sporangiophore axis. Can J Bot 48:95–109CrossRefGoogle Scholar
  12. 12.
    Gruen HE, Ootaki T (1972) Regeneration on Phycomyces sporangiophores. III. Grafting of sporangiophore segments, weight changes, and protoplasmic streaming in relation to regeneration. Can J Bot 50:139–158CrossRefGoogle Scholar
  13. 13.
    Ootaki T, Fischer EP, Lockhart P (1974) Complementation between mutants of Phycomyces with abnormal phototropism. Mol Gen Genet 131:233–246CrossRefGoogle Scholar
  14. 14.
    Ootaki T, Kinno T, Yoshida K, Eslave AP (1977) Complementation between Phycomyces mutants of mating type (+) with abnormal phototropism. Mol Gen Genet 152:245–251CrossRefGoogle Scholar
  15. 15.
    Alvarez MI, Ootaki T, Eslava AP (1983) Mutants of Phycomyces with abnormal phototropism induced by ICR-170. Mol Gen Genet 191:507–511CrossRefGoogle Scholar
  16. 16.
    Ootaki T, Lighty AC, Delbrück M (1973) Complementation between mutants of Phycomyces deficient with respect to carotenogenesis. Mol Gen Genet 121:57–70CrossRefGoogle Scholar
  17. 17.
    Koga K, Ootaki T (1983) Complementation analysis among piloboloid mutants of Phycomyces blakesleeanus. Exp Mycol 7:161–169CrossRefGoogle Scholar
  18. 18.
    Delbrück M, Ootaki T (1979) An unstable nuclear gene in Phycomyces. Genetics 92:27–48PubMedPubMedCentralGoogle Scholar
  19. 19.
    Fukui J, Miyazaki A, Ootaki T (2000) Isolation and characterization of chlorate resistance mutants from nitrate-nonutilizing fungus Phycomyces blakesleeanus. Mycoscience 41:633–640CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biological Sciences, Faculty of Science and EngineeringIshinomaki Senshu UniversityIshinomakiJapan
  2. 2.Tohoku UniversitySendaiJapan

Personalised recommendations