Phototropism pp 199-206 | Cite as
Grafting as a Tool for Study of Phototropism in Phycomyces
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Abstract
Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate. Here, we present a method of grafting sporangiophores from different mutants to obtain heterokaryotic regenerates at the graft union, based on the large size of the sporangiophore and its remarkable regeneration capability.
Key words
Complementation Fungi Grafting Phycomyces SporangiophoreReferences
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