Phototropism pp 199-206 | Cite as

Grafting as a Tool for Study of Phototropism in Phycomyces

  • Atsushi MiyazakiEmail author
  • Tamotsu Ootaki
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)


Physiological responses, such as phototropism and carotenogenesis, are usually exhibited through a specific cascade composed of several gene functions. The number of such gene functions can be determined by means of complementation analysis. For this purpose, a procedure is needed to produce heterokaryons easily and with a high success rate. Here, we present a method of grafting sporangiophores from different mutants to obtain heterokaryotic regenerates at the graft union, based on the large size of the sporangiophore and its remarkable regeneration capability.

Key words

Complementation Fungi Grafting Phycomyces Sporangiophore 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biological Sciences, Faculty of Science and EngineeringIshinomaki Senshu UniversityIshinomakiJapan
  2. 2.Tohoku UniversitySendaiJapan

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