Physiological Characterization of Phototropism in Arabidopsis Seedlings

  • Ken HagaEmail author
  • Taro Kimura
Part of the Methods in Molecular Biology book series (MIMB, volume 1924)


To date, many mutants have been isolated from dicot plants, including Arabidopsis thaliana, and the physiological roles of the isolated genes have been identified. Molecular genetic analyses have usually been conducted in the model plant Arabidopsis to identify blue-light photoreceptors and key signaling components in phototropic responses. Despite these investigations, several molecular mechanisms involved in phototropism remain unknown, possibly because detailed physiological analyses have not been conducted properly in the isolated mutants. This chapter describes an approach for the detailed investigation of hypocotyl and root phototropism in Arabidopsis seedlings. The information provided here is expected to facilitate the analysis of phototropic responses in other plant species.

Key words

Arabidopsis Dicot Hypocotyl Root Phototropism Fluence-response curve 



We thank Professor Moritoshi Iino (Osaka City University) and the members of his laboratory for providing useful technical advice, and Professor Tatsuya Sakai (Niigata University) for providing useful comments and technical support. This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (No. 24657027 and No. 17K07451 to K. H., No. 16J01942 to T. K.) and (in part) by a Grant for Basic Science Research Projects from the Sumitomo Foundation to K. H.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Applied Chemistry, Faculty of Fundamental EngineeringNippon Institute of TechnologyMiyashiroJapan
  2. 2.Graduate School of Science and TechnologyNiigata UniversityNiigataJapan

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