Identifying Protein-DNA and Protein-Protein Interactions in Avian Embryos
The chick embryo is a powerful model for experimental embryology due to its accessibility, sturdiness, and ease of manipulation. Here we describe protocols for analysis of protein-DNA and protein-protein interactions in tissues and cells isolated from the developing chick. These assays are aimed at the identification of interactions between transcription factors and regulatory elements in the genome, and, in combination with functional assays, can be used for the delineation of gene regulatory circuits.
Key wordsChick embryo Protein-DNA interactions Protein-protein interactions Chromatin precipitation (ChIP) Co-immunoprecipitation (Co-IP)
- 1.Davidson E (2009) The regulatory genome: gene regulatory networks in development and evolution. Elsevier, Burlington, MAGoogle Scholar