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Reconstitution of Intracellular Calcium Signaling in Xenopus Egg Extracts

  • Alexander A. TokmakovEmail author
  • Ken-Ichi Sato
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1920)

Abstract

Generation of calcium signal in the cytoplasm of fertilized or parthenogenetically activated eggs has been extensively studied in the intact eggs of several biological species. Calcium transient was found to elicit a plethora of biochemical and cellular events in these cells. Remarkably, intracellular calcium signaling can also be reconstituted in cell-free environment. In this chapter, we describe the methods that allow reconstitution, detection, and quantification of the calcium signal in cell-free extracts of Xenopus oocytes and eggs.

Key words

Calcium Signal transduction Xenopus laevis Egg Oocyte Cell-free extracts 

Notes

Acknowledgments

This work was supported by the Collaboration Research grant 281027 from Kobe University to A.A.T. and the Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology of Japan 15K07083 to K-I.S.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratory of Cell Signaling and Development, Department of Molecular Biosciences, Faculty of Life SciencesKyoto Sangyo UniversityKyotoJapan

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