Optimized Protocol of Zebrafish Somatic Cell Nuclear Transfer (SCNT)

  • Sukumal Prukudom
  • Kannika Siripattarapravat
  • William Poulos
  • Jose B. CibelliEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1920)


Zebrafish (Danio rerio) is an established animal model to study developmental biology as well as a wide array of human diseases. Here we describe a protocol for somatic cell nuclear transfer (SCNT). This protocol can be used to introduce genetic modifications in zebrafish and for the study of cell plasticity.

Key words

Zebrafish Somatic cell nuclear transfer Cloning 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Sukumal Prukudom
    • 1
    • 2
    • 4
  • Kannika Siripattarapravat
    • 1
    • 2
    • 3
    • 4
  • William Poulos
    • 5
  • Jose B. Cibelli
    • 5
    • 6
    Email author
  1. 1.Center for Agricultural Biotechnology, Kasetsart UniversityNakhon PathomThailand
  2. 2.Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE)BangkokThailand
  3. 3.Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart UniversityBangkokThailand
  4. 4.Kasetsart UniversityBangkokThailand
  5. 5.Department of Animal ScienceMichigan State UniversityEast LansingUSA
  6. 6.Large Animal Clinical Sciences DepartmentMichigan State UniversityEast LansingUSA

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