Generation of Xenopus Haploid, Triploid, and Hybrid Embryos
Frog species of the genus Xenopus are widely used for studies of cell and developmental biology, and recent genome sequencing has revealed interesting phylogenetic relationships. Here we describe methods to generate haploid, triploid, and hybrid species starting from eggs and sperm of Xenopus laevis and Xenopus tropicalis that enable investigation of how genome size and content affect physiology at the organismal, cellular, and subcellular levels.
Key wordsXenopus Xenopus laevis Xenopus tropicalis Fertilization Embryology Ploidy Haploid Triploid Hybridization Hybrid
We thank members of the Heald lab for input. We thank Maiko Kitaoka and Kelly Miller for critical reading of the manuscript. RG was supported by an HFSP long-term fellowship LT 0004252014-L. RH was supported by NIH R35 GM118183 and the Flora Lamson Hewlett Chair.
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