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Differentiation of Neural Stem Cells Derived from Induced Pluripotent Stem Cells into Dopaminergic Neurons

  • Marcel M. DaadiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1919)

Abstract

Dopaminergic (DA) neurons are involved in many critical functions within the central nervous system (CNS), and dopamine neurotransmission impairment underlies a wide range of disorders from motor control deficiencies, such as Parkinson’s disease (PD), to psychiatric disorders, such as alcoholism, drug addictions, bipolar disorders, schizophrenia and depression. Neural stem cell-based technology has potential to play an important role in developing efficacious biological and small molecule therapeutic products for disorders with dopamine dysregulation. Various methods of differentiating DA neurons from pluripotent stem cells have been reported. In this chapter, we describe a simple technique using dopamine-inducing factors (DIFs) to differentiate neural stem cells (NSCs), isolated from induced pluripotent stem cells (iPSCs) into DA neurons.

Key words

Self-renewable neural stem cells iPSCs Dopaminergic neuron differentiation 

Notes

Acknowledgment

This work was supported by NeoNeuron LLC.

Disclosures: Dr. Marcel M. Daadi is founder of the biotech company NeoNeuron.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Southwest National Primate Research CenterTexas Biomedical Research InstituteSan AntonioUSA
  2. 2.Department of Radiology, Research Imaging Institute, Cell Systems and Anatomy, Long School of MedicineUniversity of Texas Health Science CenterSan AntonioUSA

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