Chemically Defined Neural Conversion of Human Pluripotent Stem Cells
Human pluripotent stem cells (hPSCs) are characterized by their ability to self-renew and differentiate into any cell type of the human body. To fully utilize the potential of hPSCs for translational research and clinical applications, it is critical to develop rigorous cell differentiation protocols under feeder-free conditions that are efficient, reproducible, and scalable for high-throughput projects. Focusing on neural conversion of hPSCs, here we describe robust small molecule-based procedures that generate neural stem cells (NSCs) in less than a week under chemically defined conditions. These protocols can be used to dissect the mechanisms of neural lineage entry and to further develop systematic protocols that produce the cellular diversity of the central nervous system at industrial scale.
Key wordsPluripotency Embryonic stem cell Induced pluripotent stem cell Neural induction Cell differentiation Culture medium Coating substrate Small molecules Pathway inhibition
We thank all our colleagues at the NIH National Center for Advancing Translational Sciences (NCATS) for their collaboration and the NIH Common Fund (Regenerative Medicine Program) for funding the Stem Cell Translation Laboratory (SCTL).
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