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Reference Transcriptome for Deriving Marmoset Induced Pluripotent Stem Cells

  • Guang Yang
  • Hyenjong Hong
  • April Torres
  • Kristen E. Malloy
  • Gourav Roy-Choudhury
  • Jeffrey Kim
  • Marcel M. DaadiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1919)

Abstract

Limited access to primary tissue from various nonhuman primate (NHP) species represents a significant unmet need that hampers progress in understanding unique cellular diversity and gene regulation of specific tissues and organs in stem cell translational research. Most comparative biology studies have been limited to using postmortem tissue usually frozen specimens with limited utility for research. The generation of induced pluripotent stem cell (iPSC) lines from somatic cells, such as adult skin or blood cells, offers an alternative to invasive and ethically controversial interventions for acquiring tissue. Pluripotent iPSCs have virtually an unlimited capacity to proliferate and differentiate into all cell types of the body. We are generating high-quality validated NHP iPSC lines to offer to scientific community and facilitate their research programs. We use the non-integrative episomal vector system to generate iPSCs from NHP skin biopsies. In this chapter we describe the validation of NHP iPSC lines by confirming pluripotency and their propensity to differentiate into all three germ layers ectoderm, mesoderm, and endoderm according to established standards and measurable limits for a set of marker genes incorporated into a scorecard.

Key words

Marmoset iPSCs Pluripotency Scorecard iPSC characterization Nonhuman primate iPSCs Regenerative medicine 

Notes

Acknowledgments

The authors thank members of the Daadi laboratory for helpful support and suggestions. This work was supported by the Worth Family Fund, The Perry & Ruby Stevens Charitable Foundation, The Robert J. Kleberg, Jr., and Helen C. Kleberg Foundation, the NIH primate center base grant (Office of Research Infrastructure Programs/OD P51 OD011133), and the National Center for Advancing Translational Sciences, National Institutes of Health, through Grant UL1 TR001120.

Disclosures: Dr. Marcel M. Daadi is founder of the biotech company NeoNeuron.

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    Yang G, Hong H, Torres A, Malloy KE, Choudhury GR, Kim J, Daadi MM (2018) Standards for deriving nonhuman primate-induced pluripotent stem cells, neural stem cells and dopaminergic lineage. Int J Mol Sci 19(9):2788  https://doi.org/10.3390/ijms19092788PubMedCentralCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Guang Yang
    • 1
  • Hyenjong Hong
    • 1
  • April Torres
    • 1
  • Kristen E. Malloy
    • 1
    • 2
  • Gourav Roy-Choudhury
    • 1
  • Jeffrey Kim
    • 1
  • Marcel M. Daadi
    • 1
    • 2
    Email author
  1. 1.Southwest National Primate Research CenterTexas Biomedical Research InstituteSan AntonioUSA
  2. 2.Department of Radiology, Research Imaging Institute, Cell Systems and Anatomy, Long School of MedicineUniversity of Texas Health Science Center at San AntonioSan AntonioUSA

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