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Generation of Neural Stem Cells from Induced Pluripotent Stem Cells

  • Marcel M. DaadiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1919)

Abstract

Neural stem cells (NSCs) are defined by three necessary but not sufficient criteria: (1) self-renewable, (2) ability to generate a large number of progeny, and (3) ability to differentiate into the principal central nervous system (CNS) cell types, neurons, astrocytes, and oligodendrocytes. There are various approaches to derive neural lineages from pluripotent stem cells. It is well recognized that the chosen method of NSC derivation is critical to answering the basic biology question under investigation, to the success rate in drug discovery and to the efficacy of the therapeutic cells intended for repairing the CNS. There are three critical attributes of NSCs: (1) well-defined and stable cellular composition, (2) consistent process of perpetuation that avoids drift in composition, and (3) stable phenotype or therapeutic activity of the NSCs or their differentiated progeny. Over the past decades, we have been continuously developing consistent processes for generating stable, multipotent self-renewable NSCs from various sources. In this chapter, we report a method to generate NSCs from induced pluripotent stem cells.

Key words

Self-renewal iPSCs Multipotent neural stem cells 

Notes

Acknowledgment

This work was supported by NeoNeuron LLC.

Disclosures: Dr. Marcel M. Daadi is founder of the biotech company NeoNeuron.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Southwest National Primate Research CenterTexas Biomedical Research InstituteSan AntonioUSA
  2. 2.Department of Radiology, Research Imaging Institute, Cell Systems and Anatomy, Long School of MedicineUniversity of Texas Health Science Center at San AntonioSan AntonioUSA

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