Method to Study the Survival Abilities of Foodborne Bacterial Pathogens Under Food Processing Conditions

  • Olivier HabimanaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1918)


The proper use of controllable atmospheric containers can facilitate investigations related to the survival abilities, and physiological states of key and emerging foodborne pathogens under recreated applicable food processing environmental conditions. Of particular note, the use of saturated salt solutions can efficiently control relative humidity in airtight containers. This chapter describes a practical experimental setup, with necessary prerequisites for exposing foodborne pathogens to simulated and relevant food processing environmental conditions. Subsequent analyses for studying cell physiology will also be suggested.

Key words

Airtight containers Relative humidity Saturated salt solution Microbial survival Microbial physiology Food processing environment simulation 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.School of Biological SciencesThe University of Hong KongHong KongPeople’s Republic of China

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