Rapid On-Site Detection and Quantification of Foodborne Pathogens Using Microfluidic Devices
The potential for foodborne infectious disease outbreaks has increased not only on a local scale but also on a regional and international scale. Simple, rapid, and accurate methods to enumerate pathogenic bacteria in food and drink are required to prevent the spread of these bacteria. Here, I describe applications of a microfluidic device for on-chip fluorescent staining and semiautomated counting of target bacteria in food samples.
Key wordsOn-site monitoring Microfluidic device On-chip reaction Culture-independent method Fluorescent staining Fluorescent antibody Fluorescence microscopy
This work was supported by the JSPS KAKENHI (Grant numbers JP26670062 and 18K11688), the Kurozumi Medical Foundation, the River Foundation, the Lake Biwa-Yodo River Water Quality Preservation Organization and the Kieikai Research Foundation.
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