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Rapid On-Site Detection and Quantification of Foodborne Pathogens Using Microfluidic Devices

  • Nobuyasu YamaguchiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1918)

Abstract

The potential for foodborne infectious disease outbreaks has increased not only on a local scale but also on a regional and international scale. Simple, rapid, and accurate methods to enumerate pathogenic bacteria in food and drink are required to prevent the spread of these bacteria. Here, I describe applications of a microfluidic device for on-chip fluorescent staining and semiautomated counting of target bacteria in food samples.

Key words

On-site monitoring Microfluidic device On-chip reaction Culture-independent method Fluorescent staining Fluorescent antibody Fluorescence microscopy 

Notes

Acknowledgments

This work was supported by the JSPS KAKENHI (Grant numbers JP26670062 and 18K11688), the Kurozumi Medical Foundation, the River Foundation, the Lake Biwa-Yodo River Water Quality Preservation Organization and the Kieikai Research Foundation.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Osaka Institute of Public HealthOsakaJapan

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