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Sequence-Specific End Labeling of Oligonucleotides (SSELO)-Based Microbial Detection

  • Tanja Kostic
  • Levente Bodrossy
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1918)

Abstract

The sequence-specific end labeling of oligonucleotides (SSELO) is an alternative labelling approach for the short-oligonucleotide diagnostic microarrays that was firstly described by Rudi and coworkers (ScientificWorldJournal 3:578–584, 2003). SSELO approach is unique in a way that it shifts the specificity determining step from hybridization to labeling, ensuring both high specificity (with careful probe design even single nucleotide polymorphisms (SNPs) can be detected) and sensitivity (detection sensitivity in the range of 0.1% relative abundance has been demonstrated) of the diagnostic system. These features make SSELO approach a perfect choice for the development of microbial diagnostic microarrays, in particular in the frame of foodborne bacterial pathogen detection.

Key words

Diagnostic microarray SSELO Labelling Parallel detection Microbial detection 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Bioresources UnitAIT Austrian Institute of TechnologyTulln an der DonauAustria
  2. 2.Environmental Genomics TeamCSIRO Oceans and AtmosphereHobartAustralia

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