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The Use of Multiplex Real-Time PCR for the Simultaneous Detection of Foodborne Bacterial Pathogens

  • Alejandro Garrido-MaestuEmail author
  • David Tomás Fornés
  • Marta Prado Rodríguez
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1918)

Abstract

Foodborne pathogens continue to be a major health issue worldwide. Culture-dependent methodologies are still considered the gold-standard to perform pathogen detection and quantification. These methods present several drawbacks, such as being time-consuming and labor-intensive. The implementation of real-time PCR has allowed to overcome these limitations and even reduce costs associated with the analyses, due to the possibility of simultaneously and accurately detecting several pathogens in one single assay, with results comparable to those obtained by classical approaches. In this chapter a protocol for the simultaneous detection of two of the most important foodborne pathogens, Salmonella spp. and Listeria monocytogenes, is described.

Key words

qPCR Multiplex Salmonella spp. L. monocytogenes invA prfA Internal Amplification Control 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Alejandro Garrido-Maestu
    • 1
    Email author
  • David Tomás Fornés
    • 2
  • Marta Prado Rodríguez
    • 1
  1. 1.Department of Life Sciences, Food Quality and Safety Research GroupInternational Iberian Nanotechnology LaboratoryBragaPortugal
  2. 2.Nestlé Research Center, Institute Food Safety and Analytical Science, Microbial and Molecular Analytics GroupLausanneSwitzerland

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