Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection

  • Francisco P. Chávez
  • Andrea CalixtoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1918)


We present a protocol for the study of inter and transgenerational behavioral responses to pathogenesis in C. elegans. Transgenerational and intergenerational effects of microbes are best studied in model organisms with short life cycles, large progenies, and quantifiable cellular and behavioral outcomes. This chapter encompasses basic techniques used to study the consequences of bacterial infection in C. elegans, including worm growth, quantification of dauer larvae, and quantification of bacterial population dynamics within individual animals. Specific methods for studying transgenerational effects and their duration are also described.

Key words

C. elegans Infection Bacterial population dynamics Dauer Transgenerational Behavior 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de CienciasUniversidad de ChileSantiagoChile
  2. 2.Facultad de Ciencias, Centro de Genómica y BioinformáticaUniversidad MayorSantiagoChile
  3. 3.Facultad de Ciencias, Centro Interdisciplinario de Neurociencia de ValparaísoUniversidad de ValparaísoValparaisoChile

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