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Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection

  • Francisco P. Chávez
  • Andrea Calixto
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1918)

Abstract

We present a protocol for the study of inter and transgenerational behavioral responses to pathogenesis in C. elegans. Transgenerational and intergenerational effects of microbes are best studied in model organisms with short life cycles, large progenies, and quantifiable cellular and behavioral outcomes. This chapter encompasses basic techniques used to study the consequences of bacterial infection in C. elegans, including worm growth, quantification of dauer larvae, and quantification of bacterial population dynamics within individual animals. Specific methods for studying transgenerational effects and their duration are also described.

Key words

C. elegans Infection Bacterial population dynamics Dauer Transgenerational Behavior 

References

  1. 1.
    Klengel T, Dias BG, Ressler KJ (2016) Models of intergenerational and transgenerational transmission of risk for psychopathology in mice. Neuropsychopharmacology 41:219–231CrossRefGoogle Scholar
  2. 2.
    Palominos MF, Verdugo L, Gabaldon C et al (2017) Transgenerational diapause as an avoidance strategy against bacterial pathogens in Caenorhabditis elegans. MBio 8:e01234–e01217CrossRefGoogle Scholar
  3. 3.
    Skinner MK (2011) Environmental epigenetic transgenerational inheritance and somatic epigenetic mitotic stability. Epigenetics 6:838–842CrossRefGoogle Scholar
  4. 4.
    Dias BG, Ressler KJ (2014) Experimental evidence needed to demonstrate inter- and trans-generational effects of ancestral experiences in mammals. BioEssays 36:919–923CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Laboratorio de Microbiología de Sistemas, Departamento de Biología, Facultad de CienciasUniversidad de ChileSantiagoChile
  2. 2.Facultad de Ciencias, Centro de Genómica y BioinformáticaUniversidad MayorSantiagoChile
  3. 3.Facultad de Ciencias, Centro Interdisciplinario de Neurociencia de ValparaísoUniversidad de ValparaísoValparaisoChile

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