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High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Structural Dynamic of Foodborne Pathogens

  • Alexis Canette
  • Julien Deschamps
  • Romain BriandetEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1918)

Abstract

The functional properties of biofilms are intimately related to their spatial architecture. Structural data are therefore of prime importance to dissect the complex social and survival strategies of biofilms and ultimately to improve their control. Confocal laser microscopy (CLM) is the most widespread microscopic tool to decipher biofilm structure, enabling noninvasive 3D investigation of their dynamics down to single cell scale. The emergence of fully automated high content screening (HCS) systems, associated with large-scale image analysis, radically amplifies the flow of available biofilm structural data. In this contribution, we present an HCS-CLM protocol used to analyze biofilm 4D structural dynamics at high throughput. Meta-analysis of the quantitative variates extracted from HCS-CLM will contribute to a better biological understanding of biofilm traits.

Key words

Confocal laser microscopy High content screening Biofilm architecture Image analysis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Alexis Canette
    • 1
    • 2
  • Julien Deschamps
    • 1
  • Romain Briandet
    • 1
    Email author
  1. 1.Micalis Institute, INRA, AgroParisTechUniversité Paris-SaclayJouy-en-JosasFrance
  2. 2.IBPS InstituteSorbonne Université, CNRS, INSERMParisFrance

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