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Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells

  • Florence Dubois-Brissonnet
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1918)

Abstract

When exposed to environmental stresses, bacteria can modulate its fatty acid composition of membrane phospholipids in order to optimize membrane fluidity. Characterization of bacterial membrane fatty acid profiles is thus an interesting indicator of cellular physiological state. The methodology described here aims to improve the recovering of biofilm cells for the characterization of their fatty acid profiles. The saponification reagent is directly applied on the whole biofilm before the removal of cells from the inert surface. In this way, maximum of the cells and their fatty acids can be recovered from the deepest layers of the biofilm.

Key words

Biofilm Membrane lipids Lipidomics Fatty acids 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.UMR Micalis, INRA, AgroParisTechUniversité Paris-SaclayJouy-en-JosasFrance

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