Analysis of mRNA, miRNA, and DNA in Bone Cells by RT-qPCR and In Situ Hybridization

  • Brice Moukengue
  • Jérôme Amiaud
  • Camille Jacques
  • Céline Charrier
  • Benjamin Ory
  • Francois LamoureuxEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


The aim of this chapter is to describe a method used to evaluate gene expression and microRNAs (miRNAs) in bone cells or tissue using Reverse transcription and quantitative Polymerase Chain Reaction (RT-qPCR), and a method to assess chromogenic in situ hybridization (CISH) on Formalin Fixed Paraffin Embedded (FFPE ) mouse bone tissue to detect both DNA and mRNA transcripts using the double digoxigenin (DIG) locked nucleic acid (LNA™) probes.

Key words

Bone Bone sarcoma Gene expression RT-qPCR Primers Chromogenic in situ hybridization Histology DNA mRNA miRNA LNA probes 



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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Brice Moukengue
    • 1
  • Jérôme Amiaud
    • 1
  • Camille Jacques
    • 1
  • Céline Charrier
    • 1
  • Benjamin Ory
    • 1
  • Francois Lamoureux
    • 1
    Email author
  1. 1.INSERM, UMR1238, Bone Sarcoma and Remodeling of Calcified Tissues, Université de Nantes, Nantes Atlantique UniversitésNantesFrance

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