Analysis of Signaling Pathways by Western Blotting and Immunoprecipitation

  • Silvia Marino
  • Aymen I. IdrisEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


This chapter describes the analysis of signaling pathways in bone cells by the use of western blotting and immunoprecipitation, including a step-by-step guide to cell culture techniques, cellular and subcellular fractionation, protein isolation, purification, measurement, electrophoretic transfer, and detection.

Key words

Signaling Western blotting Immunoprecipitation Protein Phosphorylation Prenylation 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Division Hematology Oncology, Department of MedicineIndiana University School of MedicineIndianapolisUSA
  2. 2.Department of Oncology and MetabolismUniversity of SheffieldSheffieldUK

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