Bone Research Protocols pp 617-629 | Cite as
Transmission Electron Microscopy of Bone
Protocol
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Abstract
Electron microscopic analysis of mineralized tissues like bone and dentin is essential for understanding of cell–cell/cell–matrix interactions, and the three-dimensional organization of these tissues. This chapter describes a few methods to process mineralized tissues obtained from different sources for ultrastructural analysis by transmission electron microscopy.
Key words
Ultrastructure Transmission electron microscopy Bone Mineralized tissueReferences
- 1.Goggin PM, Zygalakis KC, Oreffo ROC, Schneider P (2016) High-resolution 3D imaging of osteocytes and computational modelling in mechanobiology: insights on bone development, ageing, health and disease. Eur Cell Mater 31:264–295CrossRefGoogle Scholar
- 2.Schwarcz HP (2015) The ultrastructure of bone as revealed in electron microscopy of ion-milled sections. Semin Cell Dev Biol 46:44–50CrossRefGoogle Scholar
- 3.Miltić Ž, Stolić A, Stojanović S, Najman S, Ignjatović N, Nikolić G, Trajanović M (2017) Instrumental methods and techniques for structural and physicochemical characterization of biomaterials and bone tissue: a review. Mat Sci Eng C Mater Biol Appl 79:930–949CrossRefGoogle Scholar
- 4.Gruber HE (1992) Adaptations of Goldner’s Masson trichrome stain for the study of undecalcified plastic embedded bone. Biotech Histochem 67:30–34CrossRefGoogle Scholar
- 5.Rungby J, Kassem M, Eriksen EF, Danscher G (1993) The von Kossa reaction for calcium deposits: silver lactate staining increases sensitivity and reduces background. Hist J 25:446–451CrossRefGoogle Scholar
- 6.Reynolds ES (1963) The use of lead nitrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208CrossRefGoogle Scholar
- 7.Everts V, Korper W, Jansen DC, Steinfort J, Lammerse I, Heera S, Docherty AJP, Beertsen W (1999) Functional heterogeneity of osteoclasts: matrix metalloproteinases participate in osteoclastic resorption of calvarial bone but not in resorption of long bone. FASEB J 13:1219–1230CrossRefGoogle Scholar
- 8.Landis WJ, Paine MC, Glimcher MJ (1978) Use of acrolein vapors for anhydrous preparation of bone tissue for electron microscopy. J Ultrastruct Res 70:171–180CrossRefGoogle Scholar
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