In Vivo Models of Mechanical Loading

  • Behzad Javaheri
  • Nathalie Bravenboer
  • Astrid D. Bakker
  • Albert van der Veen
  • Roberto Lopes de Souza
  • Leanne Saxon
  • Andrew A. PitsillidesEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


The skeleton fulfils its mechanical functions through structural organization and material properties of individual bones. It is stated that both cortical and trabecular morphology and mass can be (re)modelled in response to changes in mechanical strains engendered by load-bearing. To address this, animal models that enable the application of specific loads to individual bones have been developed. These are useful in defining how loading modulates (re)modeling and allow examination of the mechanisms that coordinate these events. This chapter describes how to apply mechanical loading to murine bones through points of articulation, which allows changes in endosteal, periosteal as well as trabecular bone to be revealed at multiple hierarchies, by a host of methodologies, including double fluorochrome labeling and computed tomography.

Key words

Mouse Mechanical loading Cortical bone Cancellous bone Adaptation 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Behzad Javaheri
    • 1
  • Nathalie Bravenboer
    • 2
    • 3
  • Astrid D. Bakker
    • 4
  • Albert van der Veen
    • 5
  • Roberto Lopes de Souza
    • 6
  • Leanne Saxon
    • 7
  • Andrew A. Pitsillides
    • 1
    Email author
  1. 1.Skeletal Biology Group, Comparative Biomedical SciencesThe Royal Veterinary CollegeLondonUK
  2. 2.Department Clinical ChemistryVU University Medical CenterAmsterdamThe Netherlands
  3. 3.Center for Bone Quality and Department of Internal Medicine, Division EndocrinologyLeiden University Medical CenterLeidenThe Netherlands
  4. 4.Department of Oral Cell BiologyAcademic Centre for Dentistry AmsterdamAmsterdamThe Netherlands
  5. 5.Department of Physics and Medical TechnologyVU University Medical CenterAmsterdamThe Netherlands
  6. 6.Departamento de ClínicaUniversidade Federal de Mato Grosso (UFMT)CuiabáBrazil
  7. 7.University College LondonLondonUK

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