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The Calvarial Injection Assay

  • Robert J. van ’t HofEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)

Abstract

This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.

Key words

Calvarial injection TRAP Bone formation Bone resorption GMA embedding 

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Ageing and Chronic DiseaseUniversity of LiverpoolLiverpoolUK

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