The Calvarial Injection Assay

  • Robert J. van ’t HofEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


This chapter describes the calvarial injection method, whereby the effect of a substance on bone is tested by subcutaneous injection over the calvarium of a mouse. This assay allows testing of the effect of substances on both bone resorption and bone formation in a relatively simple in vivo model. The analysis is carried out by histological means, usually in glycolmethacrylate-embedded tissue, allowing for histochemical analysis and for a variety of different histological staining methods which are also described in detail.

Key words

Calvarial injection TRAP Bone formation Bone resorption GMA embedding 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Ageing and Chronic DiseaseUniversity of LiverpoolLiverpoolUK

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