Mouse Models of Melanoma Bone Metastasis
Melanomas are aggressive cancers of the skin with high metastatic capacity. Mouse models are necessary to delineate the mechanisms of cancer metastasis and xenograft models can also allow examining the role of the host using different genetically-modified mouse models. In this chapter, I provide a detailed protocol for the preparation and inoculation of tumor cells intra-cardially and intra-tibially to achieve bone metastasis.
Key wordsBone metastasis Melanoma B16 cells Firefly luciferase Imaging
I would like to acknowledge Dr. Katherine Weilbaecher, whose lab I learned all these techniques in during my graduate studies. Specific thanks go to my fellow labmates Dr. Angela Hirbe and Dr. Elizabeth Morgan.
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