Murine Models of Bone Sarcomas

  • Camille Jacques
  • Nathalie Renema
  • Benjamin Ory
  • Carl R. Walkley
  • Agamemnon E. Grigoriadis
  • Dominique HeymannEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


This chapter describes the procedures for inducing bone sarcoma in mice. Two models based on inoculation of cancer cells in paraosseous and intraosseous site will be described. In addition to providing technical aspects of anesthesia and surgical options, key information of cell preparation and postoperative follow-up will be discussed.

Key words

Bone sarcoma Osteosarcoma Ewing sarcoma Chondrosarcoma Cell-injection Murine preclinical models Genetically-engineered mouse models 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Camille Jacques
    • 5
  • Nathalie Renema
    • 1
  • Benjamin Ory
    • 5
  • Carl R. Walkley
    • 2
  • Agamemnon E. Grigoriadis
    • 3
  • Dominique Heymann
    • 4
    Email author
  1. 1.INSERM, U1238, University of NantesNantesFrance
  2. 2.Department of Medicine, St. Vincent’s Institute of Medical ResearchSt. Vincent’s Hospital, University of MelbourneMelbourneAustralia
  3. 3.Centre for Craniofacial and Regenerative BiologyKing’s College London Guy’s HospitalLondonUK
  4. 4.Institut de Cancérologie de l’Ouest, INSERM, UMR1232, Université de Nantes, Université d’Angers, Tumour Heterogeneity and Precision MedicineNantesFrance
  5. 5.INSERM, UMR1238, Bone Sarcoma and Remodeling of Calcified Tissues, Université de Nantes, Nantes Atlantique UniversitésNantesFrance

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