Ex-Vivo Model Systems of Cancer-Bone Cell Interactions

  • Erdjan SalihEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1914)


This chapter elaborates on the state-of-the-art experimental procedures utilized in ex-vivo model systems of cancer-bone cell interactions under “static and dynamic” culture conditions and their potential use to understand cellular and molecular mechanisms as well as drug testing and discovery. An additional focus of this chapter is to provide details of how to incorporate varying oxygen tension, viz., hypoxic, normoxic, and hyperoxic, in such studies and regulate the bone biology toward dissociation of the bone remodeling stages to achieve only “bone resorption” or “bone formation” individually.

Key words

Ex-vivo model systems Bone organ cultures Static and dynamic cancer-bone co-cultures Roller-tube model Three-dimensional models Bone resorption and formation 



The author acknowledges the support of the Department of Periodontology Henry M. Goldman School of Dental Medicine Boston University.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Periodontology, Henry M. Goldman School of Dental MedicineBoston University Medical CenterBostonUSA

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