OVA-Induced Allergic Airway Inflammation Mouse Model
Asthma is a worldwide public health issue, affecting the sufferer’s quality of life. Many researchers are extensively studying the cellular processes involved in the affected airways. Experimental asthma using animals has been performed for a long time, mainly applying murine models due to well-known advantages. The aim of this study is to present an allergic airway inflammation protocol in mice. Basically, the allergic airway inflammation is induced by intraperitoneal sensitization and intratracheal challenge with ovalbumin (OVA). The model provided here mimics acute asthma characteristics including excessive mucus production, airway hyperresponsiveness, and eosinophilic airway inflammation.
Key wordsAllergy Asthma Ovalbumin Airway inflammation
This study was funded by São Paulo Research Foundation (FAPESP), project number 2012/50410-8 to CMF; 2012/50336-2 and 2016/50484-2 to FAO. CNPq (The National Council for Scientific and Technological Development), project number 486037/2012-6 to CMF. Also, the authors would like to acknowledge the fellowships provided by the Coordenação de Aperfeiçoamento Pessoal de Nível Superior - Brasil (CAPES) -Finance Code 001 to VRS and São Paulo Research Foundation (FAPESP), project number 2016/13496-2 to MCC.
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