OVA-Induced Allergic Airway Inflammation Mouse Model

  • Mateus Casaro
  • Vanessa R. Souza
  • Fernando A. Oliveira
  • Caroline M. Ferreira
Part of the Methods in Molecular Biology book series (MIMB, volume 1916)


Asthma is a worldwide public health issue, affecting the sufferer’s quality of life. Many researchers are extensively studying the cellular processes involved in the affected airways. Experimental asthma using animals has been performed for a long time, mainly applying murine models due to well-known advantages. The aim of this study is to present an allergic airway inflammation protocol in mice. Basically, the allergic airway inflammation is induced by intraperitoneal sensitization and intratracheal challenge with ovalbumin (OVA). The model provided here mimics acute asthma characteristics including excessive mucus production, airway hyperresponsiveness, and eosinophilic airway inflammation.

Key words

Allergy Asthma Ovalbumin Airway inflammation 



This study was funded by São Paulo Research Foundation (FAPESP), project number 2012/50410-8 to CMF; 2012/50336-2 and 2016/50484-2 to FAO. CNPq (The National Council for Scientific and Technological Development), project number 486037/2012-6 to CMF. Also, the authors would like to acknowledge the fellowships provided by the Coordenação de Aperfeiçoamento Pessoal de Nível Superior - Brasil (CAPES) -Finance Code 001 to VRS and São Paulo Research Foundation (FAPESP), project number 2016/13496-2 to MCC.


  1. 1.
    Global Initiative for Asthma (GINA) (2016) Global strategy for asthma management and prevention.
  2. 2.
    Kallos P, Kallos L (1984) Experimental asthma in guinea pigs revisited. Int Arch Allergy Appl Immunol 73(1):77–85CrossRefGoogle Scholar
  3. 3.
    Kips JC, Anderson GP, Fredberg JJ, Herz U, Inman MD, Jordana M et al (2003) Murine models of asthma. Eur Respir J 22(2):374–382CrossRefGoogle Scholar
  4. 4.
    Lambrecht BN, Hammad H (2015) The immunology of asthma. Nat Immunol 16(1):45–56. Scholar
  5. 5.
    Walter MJ, Holtzman MJ (2005) A centennial history of research on asthma pathogenesis. Am J Respir Cell Mol Biol 32(6):483–489CrossRefGoogle Scholar
  6. 6.
    Quarcoo D, Pavlovic S, Joachim RA (2009) Stress and airway reactivity in a murine model of allergic airway inflammation. Neuroimmunomodulation 16(5):318–324CrossRefGoogle Scholar
  7. 7.
    Holgate ST, Wenzel S, Postma DS, Weiss ST, Renz H, Sly PD (2015) Asthma. Nat Rev Dis Primers 1:15025. Scholar
  8. 8.
    Corazza N, Kaufmann T (2012) Novel insights into mechanisms of food allergy and allergic airway inflammation using experimental mouse models. Allergy 67(12):1483–1490PubMedGoogle Scholar
  9. 9.
    Reddy AT, Lakshmi SP, Reddy RC (2012) Murine model of allergen induced asthma. J Vis Exp (63):e3771.

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Mateus Casaro
    • 1
  • Vanessa R. Souza
    • 1
  • Fernando A. Oliveira
    • 2
    • 3
  • Caroline M. Ferreira
    • 1
  1. 1.Department of Pharmaceutical Sciences, Institute of Environmental, Chemistry and Pharmaceutical SciencesUniversidade Federal de São PauloDiademaBrazil
  2. 2.Cellular and Molecular Neurobiology Laboratory (LaNeC), Center for Mathematics, Computing and Cognition (CMCC)Federal University of ABC–UFABCSão Bernardo do CampoBrazil
  3. 3.Department of NeuroscienceCarleton UniversityOttawaCanada

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