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Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR/Cas9

  • Weihang Gu
  • Dabing Zhang
  • Yiping Qi
  • Zheng YuanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1917)

Abstract

Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both long-day and short-day conditions. The isolation and backcross process used for this could easily last for more than 3 years with a very low success rate. Here, we describe a straightforward method for generating csa-based PGMS lines by using the CRISPR-Cas9 technology in rice.

Key words

Photoperiod-sensitive genic male sterility (PGMS) Two-line hybrid rice Carbon starved anther (csaCRISPR-Cas9 Agrobacterium-mediated transformation Transgene-free 

Notes

Acknowledgments

This work was supported by the funds from the National Transgenic Major Program (2016ZX08009003-003-007) and National Key Research and Development Program of China (2016YFD0101107) to ZY.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Weihang Gu
    • 1
  • Dabing Zhang
    • 1
    • 2
  • Yiping Qi
    • 3
    • 4
  • Zheng Yuan
    • 1
    Email author
  1. 1.State Key Laboratory of Hybrid Rice, Shanghai Jiao Tong University–University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina
  2. 2.Plant Genomics Center, School of Agriculture, Food and WineUniversity of AdelaideUrrbraeAustralia
  3. 3.Department of Plant Science and Landscape ArchitectureUniversity of MarylandCollege ParkUSA
  4. 4.Institute for Bioscience and Biotechnology ResearchUniversity of MarylandRockvilleUSA

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