Genome Editing in Citrus Tree with CRISPR/Cas9
CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be created by Agrobacterium-mediated epicotyl transformation.
Key wordsCas9/sgRNA Genome editing Citrus Agrobacterium-mediated epicotyl transformation CsLOB1
This work has been supported by Florida Citrus Initiative.