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Genome Editing in Citrus Tree with CRISPR/Cas9

  • Hongge Jia
  • Xiuping Zou
  • Vladimir Orbovic
  • Nian WangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1917)

Abstract

CRISPR/Cas9 has been widely employed to edit genome in most of the organisms, including animal, plant, fungus, and microbe. Here we describe the modification of citrus gene CsLOB1 in transgenic citrus by Cas9/sgRNA, a two-component system derived from CRISPR-Cas9. Transgenic citrus plants can be created by Agrobacterium-mediated epicotyl transformation.

Key words

Cas9/sgRNA Genome editing Citrus Agrobacterium-mediated epicotyl transformation CsLOB1 

Notes

Acknowledgement

This work has been supported by Florida Citrus Initiative.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Hongge Jia
    • 1
  • Xiuping Zou
    • 1
  • Vladimir Orbovic
    • 2
  • Nian Wang
    • 1
    Email author
  1. 1.Citrus Research and Education Center, Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences (IFAS)University of FloridaLake AlfredUSA
  2. 2.Citrus Research and Education Center, Institute of Food and Agricultural Sciences (IFAS)University of FloridaLake AlfredUSA

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