Calpain pp 261-274 | Cite as

Genetic Models of Calpain Deficiency and Ectopic Expression

  • Yan Gao
  • Christine Hall
  • James MacLeod
  • Peter A. GreerEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1915)


Genetic manipulation in cell and animal models can provide important insights into gene function and the relationships between gene mutation and disease. This chapter describes methods to generate models of calpain-1 and calpain-2 deficiency, or their recombinant ectopic expression in cultured cells, and to genotype a conditional transgenic mouse model of calpain-1/calpain-2 deficiency that can be used to explore physiologic roles for these calpains.

Key words

shRNA knockdown CRISPR-Cas9 knockout Lentiviral vectors Conditional Cre/loxP capns1 knockout mice Calpain capn1 capn2 



This work was supported by a grant from the Canadian Institutes of Health Research (81189) to PAG.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Yan Gao
    • 1
    • 2
  • Christine Hall
    • 1
    • 2
  • James MacLeod
    • 1
    • 2
  • Peter A. Greer
    • 1
    • 2
    Email author
  1. 1.Department of Pathology and Molecular MedicineQueen’s UniversityKingstonCanada
  2. 2.Division of Cancer Biology and GeneticsCancer Research InstituteKingstonCanada

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